Pemanfaatan Zebrafish sebagai Model High-Throughput Screening untuk Penemuan dan Pengembangan Obat Anti-inflamasi
Keywords:
zebrafish, inflammatory model, drug discovery, high-throughput screeningAbstract
The development of anti-inflammatory drugs requires test models capable of presenting comprehensive immune responses, and zebrafish have become one of the most widely used animal models due to their mammalian-like immune system and compatibility with high-throughput in vivo screening. This study aims to identify, review, and compare various methods for inducing inflammation in zebrafish through a literature analysis of PubMed, ScienceDirect, SpringerLink, MDPI, and Google Scholar over the past ten years using keywords related to “zebrafish,” “inflammation,” “inflammation model,” “high-throughput screening,” “drug discovery,” and “anti-inflammatory agents.” Three main approaches to inducing inflammation in zebrafish were identified: wounding-induced, chemically-induced, and mutation-induced. Chemically induced models are the most commonly applied because they are easy to standardize and suitable for testing large numbers of compounds, whereas wounding-induced models enable real-time observation of leukocyte migration, and mutation-induced models are used to investigate chronic inflammation and specific molecular pathways. Overall, zebrafish represent an effective and relevant biological model for inflammation research and drug discovery, with diverse induction approaches that enhance sensitivity, screening throughput, and the capacity to study cellular mechanisms and pharmacological effects, thereby potentially accelerating the preclinical development of anti-inflammatory drugs.
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