Uji aktivitas antibakteri ekstrak etanol kulit salak (Salacca zalacca) terhadap pertumbuhan Staphylococcus aureus
Keywords:
antibacterial, Staphylococcus aureus, diffusion methodAbstract
Salak is a fruit which is quite popular in the society and used for diarrhea, but until now the using of salak still as food only. The other parts such as salak’s peel has not been used properly and thrown away. The aim of this study is to determine the antibacterial activity in the salak ethanolic extract against the growth of Staphylococcus aureus. Salak peel was extracted using 70% ethanol solvent using maceration method. Antibacterial activity of salak ethanol extract was tested against Staphylococcus aureus using paper disc method by using concentration exctract is 10%, 20%, 40%, and 80%. The result indicated that salak ethanolic extract can inhibit the growth of Staphylococcus aureus. The greater concentration of the extract, the clearer diameter of the cleared zone. The largest inhibitory zone is at 80% concentration with a diameter of 9.83 mm and has an equivalent to 25μg amoxicillin activity of 77.58%. Secondary metabolite of salak peel extract are alkaloids, flavonoids, polyphenols, tannins, and quinones.
References
Anisah, Yanti, A.H., dan Khotimah, S., 2014, Aktivitas Antibakteri Ekstrak Rimpang Jeringau (Acorus calamus L.) terhadap Pertumbuhan Staphylococcus aureus dan Escherichia coli, Jurnal Probiont Vol.3 No.3, 2.
Ashadi, Mulyani, B., Rahmawati, C.P., dan Setyowati, W.A.E, 2014, Skrining Fitokimia dan Identifikasi Komponen Utama Ekstrak Metanol Kulit Durian (Durio zibethinus Murr.) Varietas Petruk, Seminar Nasional Kimia dan Pendidikan Kimia VI, 275.
Brooks, G.F., dan Carroll, K.C., 2013, Bakteriologi in Jawetz, Melnick, & Adelberg, Mikrobiologi Kedokteran, Edisi 25, Penerbit Buku Kedokteran ECG, Jakarta, 194, 197.
Fatimawali, Kanon, M.Q., dan Bodhi, W., 2012, Uji Efektivitas Ekstrak Kulit Buah Salak (Salacca zalacca (Gaertn.) Voss) terhadap Penurunan Kadar Gula Darah Tikus Putih Jantan Galur Wistar (Rattus norvegicus L.) yang Diinduksi Sukrosa, Jurnal Pharmacon Vol.1 No.2, 53.
Haryati, N.A., Saleh, C., dan Erwin, 2015, Uji Toksisitas dan Aktivitas Antibakteri Ekstrak Daun Merah Tanaman Pucuk Merah (Syzygium myrtifolium Walp.) Terhadap Bakteri Staphylococcus aureus dan Escherichia coli, Jurnal Kimia Mulawarman Vol.13 No.1, 37.
Khotimah, K., 2016, Skrining Fitokimia dan Identifikasi Metabolit Sekunder Senyawa Karpain pada Ekstrak Metanol Daun Carica pubescens Lenne & K. Koch dengan LC/MS (Liquid Chromatograph-tandem Mass Spectrometry), Skripsi, Program Sarjana Sains Universitas Islam Negeri Maulana Malik Ibrahim, Malang, 44, 46.
Lestari, F., Aminah, S., dan Fitrianingsih, S.P., 2014, Uji Efek Antioksidan Ekstrak Etanol Kulit Buah Salak [Salacca Zalacca (Gaertner) Voss] dengan Metode Perendaman DPPH, Prosiding Seminar Nasional Penelitian dan PKM Sains, Teknologi, dan Kesehatan 2014, 53.
Marliana, S.D., Suryanti, V., dan Suyono, 2005, Skrining Fitokimia dan Analisis Kromatografi Lapis Tipis Komponen Buah Labu Siam (Sechium edule Jacq. Swartz.) dalam Ekstrak Etanol, Jurnal Biofarmasi, Vol. 3 No. 1, 29.
Nurina, C.I.E., Iswandi, dan Samingan, 2014, Uji Antimikroba Ekstrak Buah Salak (Salacca edulis) terhadap Bakteri Escherichia coli, Jurnal Biologi Edukasi Edisi 12 Vol.6 No.1, 19.
Pan, X., Fenqin C., Tianxing W., Honggang T., and Zhayun Z., 2009, The acid bile tolerance and antimicrobial property of Lactobacillus acidophilus NIT, Journal Food Control, Volume 20, 599.
Pratiwi, A.E, 2015, Isolasi, Seleksi dan Uji Aktivitas Antibakteri Mikroba Endofit dari Daun Tanaman Garcinia benthami Pierre Terhadap Staphylococcus aureus, Bacillus subtilis, Eschrichia coli, Shigella dysenteriae, dan Salmonella typhimurium, Skripsi, Program Sarjana Farmasi Universitas Islam Negeri Syarif Hidayatullah Jakarta, Jakarta, 35.
Salim, H.H.U, 2016, Pengaruh Aktivitas Antimikroba Ekstrak Bawang Putih (Allium sativum) terhadap Bakteri Gram Positif (Staphylococcus aureus) dan Gram Negatif (Escherichia coli) secara In Vitro, Skripsi, Program Sarjana Kedokteran Universitas Lampung, Lampung, 1.
Sholihah, I., 2015, Pengujian Aktivitas Antibakteri Ekstrak Etanol dan Infusa Daun Kupu-Kupu (Bauhinia variegate) Terhadap Bakteri Streptococcus pyogenes, Naskah Publikasi Universitas Muhammadiyah Surakarta, 8.
Wardhani, R.A.P dan Supartono, 2015, Uji Aktivitas Antibakteri Ekstrak Kulit Buah Rambutan (Nephelium lappaceum) pada Bakteri, Indonesian Journal of Chemical Science, Vol. 4 No. 1.
Downloads
Published
How to Cite
Issue
Section
License
Copyright (c) 2024 Putri Y. Nurjanah
This work is licensed under a Creative Commons Attribution-ShareAlike 4.0 International License.
This work is licensed under the Creative Commons Attribution-ShareAlike 4.0 International License.
You are free to:
- Share — copy and redistribute the material in any medium or format for any purpose, even commercial purposes.
- Adaptation — mixing, changing, and developing materials for any purpose, even commercial ones.
Under the following terms:
- Attribution — You must give appropriate credit , provide a link to the license, and indicate if changes were made . You may do so in any reasonable manner, but not in any way that suggests the licensor endorses you or your use.
- ShareAlike — If you remix, transform, or build upon the material, you must distribute your contributions under the same license as the original.
- No additional restrictions — You may not apply legal terms or technological measures that legally restrict others from doing anything the license permits.
