Pengaruh fermentasi bakteri asam laktat terhadap toksisitas ekstrak daun Nypa fruticans melalui Brine Shrimp Lethal Test (BSLT)

Aulia Azzam Jihad

Authors

Aulia Azzam Jihad

Keywords:

Nypa, fermentation, toxicity, BSLT

Abstract

Nypa is a palm-shaped mangrove plant whose leaves, fronds and fruit have many benefits. Nipah leaf extract (Nypa fruticans) has various high antioxidant contents and is also antibacterial. However, until now its use has not been maximized due to the toxic nature of the secondary metabolite content of saponin. This research aims to determine the effect of lactic acid bacteria on the extraction of nipah leaves on the toxicity of the extract. The methods used include lactic acid fermentation and toxicity tests. Fermentation by using Lactobacillus plantarum with variations in leaf and water composition of 1:2, 1:4, and 1:8 respectively for 36 hours at 42 oC. The toxicity test for fermented products was carried out using the Brine Shrimp Lethal Test (BSLT) method. The results showed that lactic acid bacterial fermentation reduced the toxicity value (LC50) of nipah leaf extract by 2.5 times.

Author Biography

Aulia Azzam Jihad

Nypa is a palm-shaped mangrove plant whose leaves, fronds and fruit have many benefits. Nipah leaf extract (Nypa fruticans) has various high antioxidant contents and is also antibacterial. However, until now its use has not been maximized due to the toxic nature of the secondary metabolite content of saponin. This research aims to determine the effect of lactic acid bacteria on the extraction of nipah leaves on the toxicity of the extract. The methods used include lactic acid fermentation and toxicity tests. Fermentation by using Lactobacillus plantarum with variations in leaf and water composition of 1:2, 1:4, and 1:8 respectively for 36 hours at 42 oC. The toxicity test for fermented products was carried out using the Brine Shrimp Lethal Test (BSLT) method. The results showed that lactic acid bacterial fermentation reduced the toxicity value (LC50) of nipah leaf extract by 2.5 times.

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